Nitrogen utilization analysis reveals the synergetic effect of arginine and urea in promoting fucoxanthin biosynthesis in the mixotrophic marine diatom Phaeodactylum tricornutum

Fucoxanthin is a new dietary ingredient applied in healthy foods with specific benefits of body weight loss and liver fat reduction. The marine diatom Phaeodactylum tricornutum highly suitable species for fucoxanthin production. In the present study, aiming to promote biosynthesis mixotrophic P. , NaNO 3 tryptone, urea were evaluated as nitrogen sources 0.10 mol L −1 glycerol organic carbon source growth shake flasks. Compared mixture tryptone (referred T+U, 1:1, N:mol N) could induce higher biomass Through utilization analysis, leucine, arginine, lysine, phenylalanine T+U medium identified amino acids that primarily support cell growth. Among those acids, arginine causes highest rate promotion. After 12 days cultivation, concentration (3.18 g ), content (12.17 mg productivity (2.68 day ) achieved using 25 mmol N 5 sources, indicating performed synergistically on enhancing pigment This study provides insights into promotion by analysis verifies synergetic effect facilitating development promising strategy efficient enhancement production through cultivation .